Nano Safe Leather

THE EFFECT ON HUMAN HEALTH OF AG/TIO2NM
TREATED LEATHERS FOR FOOTWEAR INDUSTRY

Results

Stage I-2015

 Abstract: The objectives of the first year of research activities were to develop the leather surfaces covered by Ag/TiO2NMs using two main techniques: the conventional one, which consists in spraying the new synthesized Ag-TiO2NMs embedded in film forming polymers (INCDTP-ICPI Division Romania) and an innovative method, by plasma and sputtering techniques (Universidade do Minho Portugal). Derivates objectives were: the selection of methods for cytotoxicological testing of Ag/TiO2NMs and leathers treated with NMs (Institute of Cellular Biology and Pathology "Nicolae Simionescu" Romania), selection of suitable conditions for tribological tests in view of wearing simulation (Montanuniversit√§t Leoben Austria). The main results were: the syntheses of 3 kinds of Ag/TiO2NMs (D1.1), physical-chemical characterization of new NMs (D1.2), plasma technology for leather surface treatment (D2.1), sputtering technology parameter settling (D2.2), cytotoxicological evaluation of Ag/TiO2NMs and leather surface wearing simulation by tribological tests.

Stage II-2016

 Abstract: The objectives of the second year of research activities were to develop leather surfaces with self protection properties by using Ag/TiO2 and Ag/N-TiO2 nanoparticles through spraying (INCDTP-ICPI Division Romania) or by sputtering (Universidade do Minho Portugal), the evaluation of NMs cytotoxicological impact on epithelial cells, the identification of cell death mechanism induced by NMs (Institute of Cellular Biology and Pathology "Nicolae Simionescu" Romania) and ontological model selection for cytotoxicological and inflammatory response of human cells (INCDTP-ICPI Division Romania). The main results were: Leathers for footwear with self protection properties (D1.3); Leather characterisation, Ag-TiO2NMs optimum content for surface functionalisation (D1.4.), Wearing characterisation and assessment of leachable Ag-TiO2NM (D1.5), Report on the in silico studies on cell morphology ( D5.1), Report on the cytotoxicity studies and intracellular signaling pathways activated by manufactured oxide nanoparticles in human skin cells and human epithelial alveolar cells (D3.1), Leathers with antibacterial, self cleaning and/or flame retardant properties for footwear manufacture (D6.1), Superhydrophobic leather surfaces (D2.3), Antimicrobial leather surfaces (D2.4); Technology for leather surface functionalization based on molecular layer deposition (D2.5).


The main achievements and outputs of NANO_SAFE_LEATHER project resulted in collaborative activities are listed as follows:
  • New Ag/TiO2NMs with advanced multifunctional properties for leather surface finishing as alternative for volatile organic compounds with non durable properties, pollutant for environment (water, soil, air) and consumers;
  • Cytotoxicological evaluation of Ag/TiO2NMs on human epitelial cells;
  • Ontological model for cytotoxicological response of human cells to Ag/TiO2NMs exposure;
  • Innovative techniques based on CD-sputtering for physical deposition of NMs on leather surface as an ecological alternative to spraying method;
  • New method based on tribological tests, adapted to leather structure and simulating the wearing behavior of leather surface covered with NMs;
  • A new transnational consortium dedicated to NMs study and evaluation risks, optimisation of industrial technologies for competitiveness increase based on safe use of NMs.

Scheme of TiO2 and N-TiO2 nanoparticles doping with Ag

Ag/TiO2 and Ag/N-TiO2 NPs with 50-64 nm size (TEM images) and photocatalytic activity in visible light (DRS spectra)

Leathers covered with Ag/TiO2 and Ag/N-TiO2 NPs, with antimicrobial resistance, self-cleaning properties and resistant to heat/fire

HaCaT keratinocyte viability under NMs exposure: non toxic effect for 10 - 400µg/ml A549 lung cell viability under NMs exposure: non toxic effect for 200-750 µg/ml
Cytotoxicological evaluation of Ag/TiO2NMs on human epitelial cells

Elaboration of ontological model for cytotoxicological effect on Ag/TiO2NMs on human epitelial cells

NANO_ SAFE _LEATHER

The project is funded under the frame of SIINN the ERA-NET on Nanosafety for a Safe Implementation of Innovative Nanoscience and Nanotechnology, 2nd Call (2013).

An ERA-NET is a coordination activity funded by the European Commission within the 7th Framework Programme 

(http://www.siinn.eu/index.html)


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